LOCUS HER2 CA (V659E) 9862 bp DNA circular SYN 25-AUG-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9862)
AUTHORS Li YM, Pan Y, Wei Y, Cheng X, Zhou BP, Tan M, Zhou X, Xia W,
Hortobagyi GN, Yu D, Hung MC
TITLE Upregulation of CXCR4 is essential for HER2-mediated tumor
metastasis.
JOURNAL Cancer Cell. 2004 Nov . 6(5):459-69.
PUBMED 15542430
REFERENCE 2 (bases 1 to 9862)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cancer
Cell. 2004 Nov . 6(5):459-69."
FEATURES Location/Qualifiers
source 1..9862
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(3..20)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(174..762)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(254..273)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(933..1793)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1556..1575
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1794..1898)
/gene="bla"
/label=AmpR promoter
primer_bind complement(1973..1992)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 2164..2543
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2544..2747
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2698..2718
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2792..2811
/label=T7
/note="T7 promoter, forward primer"
promoter 2792..2810
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(7344..7362)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(7345..7362)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(7382..7399)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 7388..7612
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 7658..8086
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7745..7764)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7955..7976
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(8095..8115)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8100..8429
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8280..8415
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8342..8361
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 8496..9290
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(8550..8569)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 9160..9179
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 9464..9585
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9501..9520)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9555..9574
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(9634..9650)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9634..9650)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9647..9669)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9658..9674
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9682..9712)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9727..9748
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
ORIGIN
1 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
61 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
121 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
181 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
241 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
301 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
361 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
421 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
481 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
541 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
601 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
661 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
721 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
781 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
841 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
901 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
961 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
1021 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
1081 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
1141 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
1201 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
1261 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
1321 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
1381 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
1441 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
1501 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
1561 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
1621 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
1681 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
1741 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
1801 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
1861 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
1921 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
1981 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
2041 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
2101 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
2161 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
2221 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
2281 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
2341 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
2401 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
2461 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
2521 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
2581 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
2641 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
2701 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta
2761 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
2821 cttggggcag ccgcgcgccc cttcccacgg ggccctttac tgcgccgcgc gcccggcccc
2881 cacccctcgc agcaccccgc gccccgcgcc ctcccagccg ggtccagccg gagccatggg
2941 gccggagccg cagtgagcac catggagctg gcggccttgt gccgctgggg gctcctcctc
3001 gccctcttgc cccccggagc cgcgagcacc caagtgtgca ccggcacaga catgaagctg
3061 cggctccctg ccagtcccga gacccacctg gacatgctcc gccacctcta ccagggctgc
3121 caggtggtgc agggaaacct ggaactcacc tacctgccca ccaatgccag cctgtccttc
3181 ctgcaggata tccaggaggt gcagggctac gtgctcatcg ctcacaacca agtgaggcag
3241 gtcccactgc agaggctgcg gattgtgcga ggcacccagc tctttgagga caactatgcc
3301 ctggccgtgc tagacaatgg agacccgctg aacaatacca cccctgtcac aggggcctcc
3361 ccaggaggcc tgcgggagct gcagcttcga agcctcacag agatcttgaa aggaggggtc
3421 ttgatccagc ggaaccccca gctctgctac caggacacga ttttgtggaa ggacatcttc
3481 cacaagaaca accagctggc tctcacactg atagacacca accgctctcg ggcctgccac
3541 ccctgttctc cgatgtgtaa gggctcccgc tgctggggag agagttctga ggattgtcag
3601 agcctgacgc gcactgtctg tgccggtggc tgtgcccgct gcaaggggcc actgcccact
3661 gactgctgcc atgagcagtg tgctgccggc tgcacgggcc ccaagcactc tgactgcctg
3721 gcctgcctcc acttcaacca cagtggcatc tgtgagctgc actgcccagc cctggtcacc
3781 tacaacacag acacgtttga gtccatgccc aatcccgagg gccggtatac attcggcgcc
3841 agctgtgtga ctgcctgtcc ctacaactac ctttctacgg acgtgggatc ctgcaccctc
3901 gtctgccccc tgcacaacca agaggtgaca gcagaggatg gaacacagcg gtgtgagaag
3961 tgcagcaagc cctgtgcccg agtgtgctat ggtctgggca tggagcactt gcgagaggtg
4021 agggcagtta ccagtgccaa tatccaggag tttgctggct gcaagaagat ctttgggagc
4081 ctggcatttc tgccggagag ctttgatggg gacccagcct ccaacactgc cccgctccag
4141 ccagagcagc tccaagtgtt tgagactctg gaagagatca caggttacct atacatctca
4201 gcatggccgg acagcctgcc tgacctcagc gtcttccaga acctgcaagt aatccgggga
4261 cgaattctgc acaatggcgc ctactcgctg accctgcaag ggctgggcat cagctggctg
4321 gggctgcgct cactgaggga actgggcagt ggactggccc tcatccacca taacacccac
4381 ctctgcttcg tgcacacggt gccctgggac cagctctttc ggaacccgca ccaagctctg
4441 ctccacactg ccaaccggcc agaggacgag tgtgtgggcg agggcctggc ctgccaccag
4501 ctgtgcgccc gagggcactg ctggggtcca gggcccaccc agtgtgtcaa ctgcagccag
4561 ttccttcggg gccaggagtg cgtggaggaa tgccgagtac tgcaggggct ccccagggag
4621 tatgtgaatg ccaggcactg tttgccgtgc caccctgagt gtcagcccca gaatggctca
4681 gtgacctgtt ttggaccgga ggctgaccag tgtgtggcct gtgcccacta taaggaccct
4741 cccttctgcg tggcccgctg ccccagcggt gtgaaacctg acctctccta catgcccatc
4801 tggaagtttc cagatgagga gggcgcatgc cagccttgcc ccatcaactg cacccactcc
4861 tgtgtggacc tggatgacaa gggctgcccc gccgagcaga gagccagccc tctgacgtcc
4921 atcatctctg cggtggaggg cattctgctg gtcgtggtct tgggggtggt ctttgggatc
4981 ctcatcaagc gacggcagca gaagatccgg aagtacacga tgcggagact gctgcaggaa
5041 acggagctgg tggagccgct gacacctagc ggagcgatgc ccaaccaggc gcagatgcgg
5101 atcctgaaag agacggagct gaggaaggtg aaggtgcttg gatctggcgc ttttggcaca
5161 gtctacaagg gcatctggat ccctgatggg gagaatgtga aaattccagt ggccatcaaa
5221 gtgttgaggg aaaacacatc ccccaaagcc aacaaagaaa tcttagacga agcatacgtg
5281 atggctggtg tgggctcccc atatgtctcc cgccttctgg gcatctgcct gacatccacg
5341 gtgcagctgg tgacacagct tatgccctat ggctgcctct tagaccatgt ccgggaaaac
5401 cgcggacgcc tgggctccca ggacctgctg aactggtgta tgcagattgc caaggggatg
5461 agctacctgg aggatgtgcg gctcgtacac agggacttgg ccgctcggaa cgtgctggtc
5521 aagagtccca accatgtcaa aattacagac ttcgggctgg ctcggctgct ggacattgac
5581 gagacagagt accatgcaga tgggggcaag gtgcccatca agtggatggc gctggagtcc
5641 attctccgcc ggcggttcac ccaccagagt gatgtgtgga gttatggtgt gactgtgtgg
5701 gagctgatga cttttggggc caaaccttac gatgggatcc cagcccggga gatccctgac
5761 ctgctggaaa agggggagcg gctgccccag ccccccatct gcaccattga tgtctacatg
5821 atcatggtca aatgttggat gattgactct gaatgtcggc caagattccg ggagttggtg
5881 tctgaattct cccgcatggc cagggacccc cagcgctttg tggtcatcca gaatgaggac
5941 ttgggcccag ccagtccctt ggacagcacc ttctaccgct cactgctgga ggacgatgac
6001 atgggggacc tggtggatgc tgaggagtat ctggtacccc agcagggctt cttctgtcca
6061 gaccctgccc cgggcgctgg gggcatggtc caccacaggc accgcagctc atctaccagg
6121 agtggcggtg gggacctgac actagggctg gagccctctg aagaggaggc ccccaggtct
6181 ccactggcac cctccgaagg ggctggctcc gatgtatttg atggtgacct gggaatgggg
6241 gcagccaagg ggctgcaaag cctccccaca catgacccca gccctctaca gcggtacagt
6301 gaggacccca cagtacccct gccctctgag actgatggct acgttgcccc cctgacctgc
6361 agcccccagc ctgaatatgt gaaccagcca gatgttcggc cccagccccc ttcgccccga
6421 gagggccctc tgcctgctgc ccgacctgct ggtgccactc tggaaaggcc caagactctc
6481 tccccaggga agaatggggt cgtcaaagac gtttttgcct ttgggggtgc cgtggagaac
6541 cccgagtact tgacacccca gggaggagct gcccctcagc cccaccctcc tcctgccttc
6601 agcccagcct tcgacaacct ctattactgg gaccaggacc caccagagcg gggggctcca
6661 cccagcacct tcaaagggac acctacggca gagaacccag agtacctggg tctggacgtg
6721 ccagtgtgaa ccagaaggcc aagtccgcag aagccctgat gtgtcctcag ggagcaggga
6781 aggcctgact tctgctggca tcaagaggtg ggagggccct ccgaccactt ccaggggaac
6841 ctgccatgcc aggaacctgt cctaaggaac cttccttcct gcttgagttc ccagatggct
6901 ggaaggggtc cagcctcgtt ggaagaggaa cagcactggg gagtctttgt ggattctgag
6961 gccctgccca atgagactct agggtccagt ggatgccaca gcccagcttg gccctttcct
7021 tccagatcct gggtactgaa agccttaggg aagctggcct gagaggggaa gcggccctaa
7081 gggagtgtct aagaacaaaa gcgacccatt cagagactgt ccctgaaacc tagtactgcc
7141 ccccatgagg aaggaacagc aatggtgtca gtatccaggc tttgtacaga gtgcttttct
7201 gtttagtttt tacttttttt gttttgtttt tttcaagctt ggtaccgagc tcggatccac
7261 tagtaacggc cgccagtgtg ctggaattct gcagatatcc atcacactgg cggccgctcg
7321 agcatgcatc tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca
7381 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
7441 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
7501 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
7561 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
7621 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
7681 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
7741 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
7801 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
7861 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
7921 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
7981 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
8041 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
8101 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
8161 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
8221 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
8281 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
8341 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
8401 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
8461 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
8521 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
8581 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
8641 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
8701 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
8761 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
8821 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
8881 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
8941 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
9001 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
9061 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
9121 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
9181 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
9241 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
9301 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
9361 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
9421 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
9481 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
9541 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
9601 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
9661 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
9721 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
9781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
9841 gcgtattggg cgctcttccg ct
//
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